Investigating the molecular mechanism of Daxueteng-Feilongzhangxue Herbal Pair in treating diabetic cognitive impairment based on network pharmacology
LOU Fangli
TIAN Hui
LUO Jie
WU Yuanhua
Abstract：Objective:To investigate the molecular mechanism of action of the traditional Chinese medicine(TCM)pair of Daxueteng-Feilongzhangxue in the treatment of diabetic cognitive impairment(DCI)based on network pharmacology.Methods:The target genes were screened based on TCMSP,HERB,and PubChem databases,and the intersection of the target genes was obtained using Venny 2.1.0.Cytoscape v3.9.1 was used to create a drug-active component-gene target network,and the PPI network diagram was established using the STRING database.DAVID database and MicroHIS were used for GO function enrichment analysis and KEGG pathway enrichment analysis,and Cytoscape v3.9.1 was used to establish a target-pathway network diagram.Results:The effective components of Daxueteng and Feilongzhangxue were 33 and 21,respectively,with a common active component of 2.The drug-disease intersection target genes were 275.The core genes included ALB,AKT1,SRC,TNF,PPARG,PRKACA,EGFR,ESR1,APP,BCL2,MAPK3,PTGS2,STAT3,CASP3,GRIN2B,GSK3β,HSP90AA1,DRD2,HIF1A,and NFKB1.The GO function enrichment analysis identified 932 terms,including phosphorylation,response to exogenous stimuli,signal transduction,negative regulation of apoptotic process,and response to hypoxia/lipopolysaccharide.The KEGG pathways were 190,concentrated in human diseases,biological and environmental information processes.They included diabetes complications,cancer,viral infections,atherosclerosis,endocrine resistance,and AGE-RAGE,HIF-1,PI3K/Akt,prolactin,Ras,Relaxin signaling pathways,etc.Conclusion:The common active constituents of Daxueteng and Feilongzhangxue in treating DCI are β-sitosterol and chlorogenic acid.The drug pair regulates target genes such as ALB,AKT1,SRC,TNF,PPARG,PRKACA,etc.by adjusting the AGE-RAGE,HIF-1,PI3K/Akt signaling pathways,cancer,diabetes complications,viral infections,atherosclerosis,endocrine resistance,and other pathways to achieve anti-cancer,treatment of diabetes complications,anti-viral infections,improvement of hemorheology,improvement of endocrine and metabolism,etc.to exert therapeutic effects on DCI.
Keywords：DaxuetengFeilongzhangxuenetwork pharmacologydiabetic cognitive impairmentmolecular mechanism of action
Publication Date：2025-02-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 664-674 )