The expression of long non-coding RNA CRNDE regulates the growth and adhesion of multiple myeloma cells by regulating the IL-6 signaling pathway
Guo Lifang
Xi Zhenfang
Gao Guorong
Hao Hua
Abstract：Objective To investigate the effect of long non-coding RNA colorectal neoplasia differentially expressed gene(CRNDE)expression on the growth and adhesion of multiple myeloma(MM)cells and its mechanism.Methods The MM cell line KMS11 was transfected with plasmids to construct a control group and a CRNDE knockout group with normal expression of CRNDE.Observe the changes in cell proliferation and adhesion in MTT assay after knocking out CRNDE,and analyze the downstream transcription factors of CRNDE using scatter plots.IL-6 stimulation was performed,with half of the culture using basic medium and the other half using medium containing 2 ng/ml IL-6.Flow cytometry was used to detect the fold decrease in mean fluorescence intensity(MFI)and observe changes in cell proliferation ability.Immunodeficient mice were selected for in vivo tumorigenicity experiments,and tumor diameters were compared between the control and CRNDE knockout groups.Results After 8 days of cultivation,the MTT cell proliferation OD value of the CRNDE knockout group was(0.32±0.12)lower than that of the control group,which was(0.65±0.16).The OD value of cell adhesion experiment in CRNDE knockout group was(0.08±0.01)lower than that in the control group(0.19±0.02),both(P＜0.001).IL-6 receptor is a downstream factor of CRNDE.The IL-6 stimulation experiment showed that there was no statistically significant difference in the fold reduction of MFI between the two groups without IL-6 medium(P＞0.05).After 6 and 8 days of cultivation in IL-6 medium,the MFI reduction fold of the RNDE knockout group and the control group were(65.29±11.58)vs.(101.25±12.37),(124.57±13.49)vs.(213.45±19.42),respectively,with(P＜0.001),indicating that knocking out CRNDE can inhibit MM cell proliferation through the IL-6 pathway.The mouse experiment showed that the tumor diameter in the CRNDE knockout group was(1.84±0.13)mm,which was lower than that in the control group(9.53±0.10)mm(t=148.26,P=0.000).Conclusion The expression of Lnc RNA CRNDE is closely related to the proliferation and adhesion of MM cells.Knocking out CRNDE can inhibit the proliferation and adhesion of MM cells and reduce their tumorigenic effects.Its mechanism of action may be related to the IL-6 signaling pathway.
Keywords：Lnc RNA CRNDEMultiple myelomaIL-6IL-6RCell growthCell adhesion
Publication Date：2025-08-31
Online Publishing Date：2025-09-24(First online date of this platform, not the publication date of the document)
Pages：4( 650-653 )