Study on expression of PLK1,MYH9 and Smac in keloid tissue

Zhao Zishen; Ding Cheng; Huang Bingtai

doi:10.3969/j.issn.1674-3245.2025.01.030

Abstract：Objective To investigate the expression patterns and correlations of PLK1,MYH9,and Smac in keloid tissues.Methods Retrospectively,97 patients who underwent keloid tissue resection in our hospital from October 2022 to October 2023 were selected as the research objects of this study.Samples of keloid tissue and paracoid normal tissue surgically removed from keloid patients were collected,and immunohistochemical markers were made after section.Furthermore,Western Blot was used to detect the expressions of PLK1 and MYH9,PT-PCR was used to detect the expression of Smac,and the expressions of PLK1,MYH9 and Smac in clinical symptoms of keloid patients were analyzed,and the risk factors of keloid occurrence were analyzed by multi-factor Logistic.Spearman correlation analysis was conducted to analyze the correlation between PLK1,MYH9,Smac and clinical features of patients,and ROC curve was used to analyze the predictive value of the combined detection of PLK1,MYH9,and SMAC in patients with keloid.Results In normal skin tissue,the expression levels of PLK1(1.13±0.15)and MYH9(0.95±0.14)were found to be lower than those in keloid tissue,where PLK1 was 1.49±0.30 and MYH9 was 1.43±0.20.The expression level of Smac(1.16±0.13)in normal skin tissue was higher than that in keloid tissue(0.62±0.09),and the difference was statistically significant(t=10.570,19.364,33.926,P<0.05).There were significant differences in the expressions of PLK1,MYH9 and Smac in family history,nationality,location of lesions,number of lesions and maximum diameter of lesions in patients with keloid(P<0.05).Logistic regression analysis showed that the expressions of PLK1,MYH9 and Smac were risk factors for keloid development(P<0.05).Spearman correlation analysis showed that PLK1,MYH9 and Smac were positively correlated with family history,nationality,location of lesions,number of lesions and maximum diameter of lesions(P<0.05).ROC curve analysis showed that the single diagnosis of PLK1,MYH9 and Smac was significantly lower than the combined diagnosis of the three,indicating that the combined detection had the highest diagnostic efficiency and had a significant difference(P<0.05).Conclusion The levels of PLK1 and MYH9 increases in keloid tissue,while Smac decreases in keloid tissue,which are closely related to the clinical characteristics of patients,and can better predicts the prognosis of keloid patients and provides theoretical basis for clinical diagnosis.