P-STAT3 expression and its relationship with Hp infection in patients with colon adenomatous polyps and colon cancer

Liu Yongjiang; Wang Juan; Liu Qiming

doi:10.3969/j.issn.1674-3245.2023.06.018

Abstract：Objective To observe the expression of tyrosine phosphorylation signaling and activators of transcription 3(P-STAT3)in colon adenomatous polyps and colon cancer tissues,and the relationship between Helicobacter pylori(Hp)infection and P-STAT3 expression.Methods 74 cases of colon adenomatous polyps admitted to our hospital from November 2019 to December 2022,67 cases of colon cancer specimens,and 50 cases of negative patients undergoing colonoscopy were collected.Immunohistochemical staining,rapid urease test or 13C urea breath test for Hp detection.Compare the positive rates of p-STAT3 expression and Hp expression in three groups,and analyze the relationship between Hp infection,characteristics of adenomatous polyps,and pathological parameters of colorectal cancer.Results The infection rates of Hp in the normal control group,adenomatous polyp group,and colon cancer group were 24.0%(12/50),68.92%(51/74),and 59.70%(40/67),respectively.The infection rates in the adenomatous polyp group and colon cancer group were higher than those in the normal group,and the differences between the groups were statistically significant(χ2 = 24.088,17.780,P<0.05).There was no statistically significant difference in the infection rates between the polyp group and the colon cancer group(χ2 =1.305,P>0.05).The proportion of polyps above 1 cm and advanced adenomas in patients with positive Hp infection in the polyp group was higher than that in patients with negative Hp infection.The difference between the groups was statistically significant(χ2 = 3.844,4.610,P<0.05).There was no statistically significant difference in gender,age,number of polyps,or location between the two subgroups(P>0.05).There was no statistically significant difference in gender,age,tumor differentiation,maximum diameter,invasion of serosa and lymph node metastasis between Hp infected and uninfected colon cancer patients(P>0.05).The positive expression rates of P-STAT3 in the normal control group,adenomatous polyp group,and colon cancer group were 8.0%(4/50),47.29%(35/74),and 85.08%(57/67),respectively.The expression of P-STAT3 gradually increased in the three groups,and the differences between the two groups were statistically significant(P<0.05).There was a positive correlation between Hp infection and P-STAS3 expression in the adenomatous polyps group and the colon cancer group(r=0.314,0.193,P<0.05).Conclusion Helicobacter pylori infection may increase the incidence rate of colon adenoma and colon cancer,which may play a role in the development of colon cancer through P-STAT3 pathway.Colonoscopy screening and early intervention should be strengthened for Hp infected individuals.