The differentially gene expression between influenza pre-vaccination and post-vaccination among frail and non-frail older adults

Fan Jiao; Zeng Jing; Shi Jingqi; Xu Guogang; Li Tianzhi

doi:10.3969∕j.issn.1674-3245.2023.05.010

Abstract：Objective Through transcriptome sequencing data analysis,explore the changes in gene expression in elderly individuals with different frailty statuses before and after receiving influenza A vaccine.Methods We downloaded transcriptome sequencing data from the GEO database and analyzed the differentially expressed genes(DEGs)between frail and non-frail groups at Days 0,3 and 7 post-vaccination.GO functional enrichment analysis and KEGG pathway analysis were used to annotate the functions of the DEGs.According to the correlation of genes,the parameter β =5 was used to construct the gene co-expression network when the correlation coefficient threshold was set to 0.85 and screened gene modules significantly associated with clinical traits.The hub genes were screened according to the connectivity within modules.Results There were 2756,1558,and 1783 differentially expressed genes between frail and non-frail older adults before vaccination and 3 and 7 days after vaccination,respectively.A total of 12 gene co-expression modules were constructed from the correlations of gene expression,in which the green modules were significantly correlated with the Age and Frailty status;We found that 17 genes including B3GAT1,PTGDR were in the hub of the co-expression network;The result of biological annotation function revealed that the genes in green modules were mainly enriched in adaptive immune response,T cell activation and natural killer cell activation pathways.Conclusion Gene expression in elderly individuals with different frailty statuses changes before and after receiving influenza A vaccine,with differences observed in immune response,T cell activation,and other signaling pathways.