Study on the effect of the cordyceps polysaccharide on the expressions of TGF-β1,MCP-1 and mRNA in rat model of chronic renal failure induced by adenine

Ren Yongfei; Hou Xiaobo

doi:10.3969/.issn.1674-3245.2017.05.016

Abstract：Objective To explore the effect of Cordyceps Polysaccharide on the protein and mRNA expressions of TGF-β1 and MCP-1 in rat model of chronic renal failure induced by adenine. Methods 20 rats from 60 SPF of clean male SD rats were randomly selected as control group. The remaining 40 rats were treated by adenine for chronic renal failure model construction. Rats of chronic renal failure model were randomly divided into model group and experimental group ,20 rats in each group. Rats in the experimental group were given Cordyceps polysaccharide 160 mg/kg by gavage,rats in the control group and model group were given normal saline,once a day,14 d. After treatment,serum creatinine (Scr),blood urea nitrogen (BUN) content were analyzed by biochemical analyzer;Renal tissue TGF-β1,MCP-1 protein content was determined by immunohistochemical analysis. The expression of TGF-β1 mRNA and MCP-1 mRNA were determined by RT-PCR. Results After treatment,serum Scr and BUN in model group and experimental group were significantly higher than in the control group,the difference was statistically significant (P < 0.05);The levels of serum Scr and BUN in experimental group were significantly lower than that of control group ,the difference was statistically significant (P < 0.05). After treatment,there was rarely expressed TGF-β1,MCP-1 in renal tissue of control group,but strong expression in renal tubular epithelial cell in renal tissue of model group and interstitial cells. There was also positive expressions of TGF-β1 and MCP-1 in renal tissue of the experimental group,but its expression levels was significantly lower than that of model group;The OD value of TGF-β1 and MCP-1 in renal tissue in the model group and the experimental group were significantly higher than in the control group,the difference was statistically significant (P<0.05). The expression of TGF-β1 mRNA in the renal tissue in model group and experimental group were significantly higher than that in control group ,and the experimental group was significantly lower than that in the model group,the difference was statistically significant (P < 0.05). The expression of MCP-1 mRNA in renal tissue of model group and experimental group were significantly higher than in control group ,and the experimental group was significantly lower than that in the model group,the difference was statistically significant (P < 0.05). Conclusion Cordyceps polysaccharide can significantly reduce the protein and mRNA expression of TGF-β1 and MCP-1 in chronic renal failure rats induced by adenine,and it also obviously improves the function of kidney. The results are worth of clinical application and promotion.