Combined detection of 3 antibodies against mycobacterium tuberculosis:evaluation of the diagnosis of senile tuberculous pleurisy using ROC curves
Abstract：Objective To detect 3 antibodies against tuberculoprotein, LAM, and 2 proteins at molecular weights of 38kD and 16kD using a protein chip and to explore its clinical value in the diagnosis of senile tuberculous pleurisy. Methods The serum antibodies(LAM,16kD and 38kD)from 126 senile patients(49 cases of tuberculous pleurisy and 77 cases of malignant pleural fluid) were analyzed using a protein chip, and the corresponding receiver operating characteristic (ROC) curves for single and combined multi-antibodies were also constructed in order to evaluate their diagnostic values for tuberculous pleurisy, which is compared with acid fast stain of pleural fluid. Results The 16kD, LAM, 38kD and, especially, the combined test, showed better diagnostic effects over acid fast stains of pleural fluid with superior sensitivity(69.39%,69.39%,69.39% over 22.45%),specificity(92.21%,100.00%, 100.00% over 97.40%),accuracy (83.33%,88.10%,88.10% over 68.25%)and area under curve (AUC)results (0.81,0.85,0.85 over 0.60)(P < 0.05). Conclusion The tuberculosis protein chip assay of LAM,16kD and 38kD is a more convenient and rapid test for the diagnosis of tuberculous pleurisy that could significantly improve the diagnostic effect.
Keywords：Protein chipAntibodyTuberculosisPleuralROC curve
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 316-318 )