Study on the value of multi-index combined detection in the diagnosis of mycobacterium tuberculosis
Li Zhensheng
Li Dexin
Li Chunxia
Abstract：Objective To study the value of sputum or bronchoalveolar lavage fluid fluorescence quantitative TB-DNA, mycobacterium tuberculosis phage detection, mycobacterium tuberculosis and T-SPOT multi-target detection in the diagnosis of pulmonary tuberculosis. Methods A total of 223 patients in our hospital were enrolled from October 2010 to October 2012. The patients were divided into three groups:the untreated, patients diagnosed with bacterium-positive pulmonary tuberculosis and patients with non-pulmonary tuberculosis. TB-DNA was detected in the blood of the selected patients using routine T-SPOT and mycobacterium tuberculosis bacteriophage tests; sputum or alveoli tests were also used. The patients' detection results were recorded and analyzed. The specificity and sensitivity values of the above three tests were analyzed, as was the combination of the three tests. Results The specificity of each item in the three tests was greater than 90%. The numbers of positive bacteria in the tuberculosis and bacterium-positive pulmonary tuberculosis groups were much larger than in the non-tuberculosis group. The number of positive bacteria detected in pulmonary tuberculosis cases also increased. The detection rate of the T-SPOT test was statistically significantly higher as well (P < 0.05), and the detection rate of the T-SPOT test was significantly higher than in the non-tuberculosis group. There was a significant difference in the positive rate of positive bacteria between the negative group and the non-pulmonary tuberculosis group(P<0.05). Conclusion The combination of the T-SPOT test, mycobacterium tuberculosis detection and TB-DNA detection can improve the diagnosis of bacterium-negative pulmonary tuberculosis. This includes the use of the T-SPOT test to check the accuracy of the combined examination;it can also improve the detection of bacterium-negative tuberculosis.
Keywords：Bacterial negative pulmonary tuberculosisT-SPOT detectionTB-DNA detectionDiagnosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 307-309 )