Application of environmental DNA technology in water ecosystem research
HUANG Qianhao
YUAN Yufan
CHEN Shaozhou
XI Mei
DU Fengfeng
LI Bei
LIANG Yu
LIU Xiaojing
YAO Dongrui
Abstract：Due to the intensification of human activities,the function of water ecosystem is degraded,and the aquatic biodiversity is seriously threatened.For aquatic ecosystems such as oceans,lakes and rivers,it is of great significance to investigate the species diversity and biomass of aquatic organisms for the monitoring and scientific evaluation of aquatic ecosystems.The environmental DNA(eDNA)can be collected from environmental samples such as water and sediment.The eDNA technology,which is a useful supplement to large-scale biodiversity survey,can effectively monitor the biological species,bio-mass and spatial-temporal distribution in the water environment by high-throughput sequencing.Due to the advantage of environmental friendliness,easy sampling and high sensitivity,eDNA technology has captured widespread attention,and it has been extensively applied in the field of aquatic ecosystem for recent years.Accordingly,this manuscript focused on the technical principle and operation process of eDNA technology.In addition,the research progress and typical cases of eDNA technology in the as-sessment of species diversity and biomass in oceans or lakes were summarized.Furthermore,the key factors affecting the resolution and universality of eDNA technology was identified.The application and development of eDNA technology in marine,lake,river and other water ecosystems were also prospec-ted,in order to provide technical reference for the application of eDNA technology in water ecosystems.
Keywords：environmental DNAwater ecosystembiological investigationbiodiversity
Publication Date：2026-02-25
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 264-270 )