Study on the characteristics and influencing factors of aerobic ammonium oxidizing archaea community in surface sediments of mangroves in Sanya River

CHENG Hao; ZHEN Yu; LUO Qing; HE Hui; ZHAO Liyuan

doi:10.13984/j.cnki.cn37-1141.2026.01.011

Abstract：As the key step of biological nitrification,the ammonia oxidation process plays an important role in the global nitrogen cycle.Based on the Illumina Miseq sequencing,we explored the characteris-tics of aerobic ammonia-oxidizing archaea(AOA)community in surface sediments of mangrove in San-ya River in winter and summer,and analyzed the key environmental factors affecting the community structure.The results showed that the AOA community had seasonal variation characteristics,the di-versity and richness of the AOA community in most sampling sites were higher in summer than in win-ter,but there was no significant difference(P＞0.05).The composition of AOA community in differ-ent sections from the upper to lower reaches and from the river to the land side of the Sanya River was different,among which the difference between the upper section and the middle with the lower section was the largest,and there was also a certain difference in the composition of AOA community in winter and summer.The dominant AOA genera in winter and summer were Nitro so pumilus,Candidatus Ni-trosocosmicus and Nitrosos phaera.Geographical location and sampling time were the main factors af-fecting the AOA community,and geographical location had a significant impact on AOA community structure(P＜0.05).The main environmental factors affecting the AOA community structure includ-ed DO,nitrate,nitrite,temperature and pH.Nitrite and temperature mainly affect the AOA commu-nity structure in winter,and temperature was significantly related to it(P＜0.05);pH mainly affected the AOA community structure in summer,but the correlation between environmental factors and it was not significant(P＞0.05).In general,the environmental factors affecting the AOA community structure in winter and summer were DO and nitrate.