Effects of fillers and hydraulic retention time on microbial community structure in the recirculating mariculture system of Sebastes schlegelii
LI Jiaxin
CUI Zhengguo
HU Haiyan
FENG Yuna
JIN Tongtong
ZHU Shengmin
Abstract：Microbial community is crucial to the biological filler tank of industrial recirculating aquacul-ture system.In order to explore the effects of the filler and hydraulic retention time in the biological fil-ter tank of recirculating aquaculture system on the microbial community structure and diversity inside,we selected valens clip,polyurethane sponge and polyethylene ring as the filler of MBBR tank and brush as the filler of FBBR tank.Four groups of hydraulic retention times were also set to run the cir-culating water system and compare the purification effect of water quality.Finally,high-throughput se-quencing technology was used to detect the microbial community structure and analyze the diversity of the filler surface biofilm.The results showed that the dominant phylum in the packing were proteobac-teria,Bacteroidetes,actinobacteria,chloroflexi and planctomycetes and there was no significant differ-ence among the samples at the class level.The dominant classes were alphaproteobacterial,bacte-roidia,gammaproteobacterial,acidimicrobiia and deltaproteobacteria,and there is a great difference a-mong the samples at the genus level.Ruegeria,Sva0996_marine_group,Planktotalea,Pseudomonas,Muricauda,and Woeseia were dominant genera with obvious changes in abundance.When the hydraulic retention time was 1.6h,polyurethane sponge showed the best removal effect on NH4+-N with an av-erage removal rate of 21.66±2.92％.When the hydraulic retention time was 2.4 h,polyethylene ring performed best in removing NO2--N with an average removal rate of 31.42±7.00％,which is mainly due to the main role of bacteria with ammoxidation and nitrification abilities.In this study,the removal effect of different fillers and the changes of microbial community structure and diversity over time in re-circulating aquaculture system were studied.The results provided a theoretical reference for microbial en-hancement and improvement of nitrogen purification efficiency in industrial recirculating aquaculture system.
Keywords：recirculating aquaculture systemmicroorganismhigh-throughput sequencinghydraulic retention time
Publication Date：2026-02-25
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 60-67 )