Influence of lipid extraction on stable carbon and nitrogen isotope analyses of muscle and liver of Scomber japonicus
ZHANG Jiangdong
CHEN Xinjun
GONG Yi
LIANG Qian
Abstract：In this study,46 Japanese mackerel(Scomber japonicus)individuals collected from the northwest Pacific were used to explore the necessity of the lipid extraction for the stable isotope analysis of Japanese mackerel,and to determine the influence of lipid extraction on the carbon and nitrogen sta-ble isotope ratios(δ13C,815N)of the muscle and liver of thie fish.The relationship between the ratio of carbon and nitrogen content(C:N)and the constant 3.50 was compared and analyzed to determine whether this species needs lipid extraction pretreatment.The δ13C correction model of muscle with liver was proposed.After lipid extraction,δ13C and δ15N of muscle increased by 0.41‰ and 0.61‰,respec-tively,while C:N decreased by 0.21,δ13C of liver increased by 0.73‰,δ15N kept unchenged and C:N decreased by 0.31.Individual analysis of muscle and liver C:N below 3.50 showed significant differ-ences in δ13C after lipid pumping.Accodingly,the relationship between C:N and constant 3.50 is not appropriate for determing whether Japanese mackerel needs a lipid extraction pretreatment.Lipid ex-traction is necessary before 813C analysis for studying Japanese mackerel muscle and liver.The optimal calibration models are Δδ13C=-0.53 × δ13Cbulk-9.85 and Δδ13C=-0.79 × δ13 Cbulk-14.88.Lipid ex-traction was not performed when δ15N of Japanese mackerel in muscle was analyzed while lipid extrac-tion was not necessary for the analysis of δ15N in Japanese mackerel liver.
Keywords：Japanese mackerellipid extractionstable isotopemuscleliver
Publication Date：2025-12-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 138-146 )