Studies on the immune pathway of Coilia nasus infected with Anisakidae nematodes

WANG Honglan; YING Congping; YANG Yanping; XU Pao; LIU Kai

doi:10.13984/j.cnki.cn37-1141.2025.04.022

Abstract：Coilia nasus is a typical anadromous migratory fish species.During offshore fattening,it is often infected by feeding on fish and shrimp parasitic with Anisakidae nematodes.The liver of C.nasus captured in Yangtze River is one of the most important attachment tissues of Anisakidae nematodes which cause visible damage to the liver of C.nasus.To screen the changes of immune proteins in the liver tissue of C.nasus caused by Anisakidae nematode infection.iTRAQ technology was used to char-acterize the changes of liver proteins of C.nasus caused by Anisakidae nematode infection,and explore the expression of immune pathway in C.nasus.Through the differential analysis of protein synthesis,a total of 339 differentially synthesized proteins were identified,of them 193 were up-regulated and 146 were down regulated.There were 37 differentially synthesized proteins relating to immunity,of them 9 were up-regulated and 28 were down regulated.GO and pathway enrichment analyses showed 10 im-mune related pathways which include phagosome,lysosome,endocytosis,autophagy animal,comple-ment and coagulation cascade and toll like receptor signaling pathway among others.Seven immune re-lated differentially synthesized proteins were identified,which include complement C3,C7 and C1q,MHC-Ⅰ,MHC-Ⅱ,Ig and rab-7a.The syntheses of these proteins were significantly down regulated in parasitic group.Anisakidae nematodes affect the complement cascade reaction and phagocytosis process of C.nasus by inhibiting the syntheses of immune related proteins,and escaping the host im-mune response.