Studies on the fingerprint characteristics of abalones based on stable isotope ratio and their origin tracing

HONG Heyang; TIAN Xiuhui; JIANG Lisheng; PENG Kaixiu; CUI Yanmei; ZHAO Junqiang; CAO Wei; QIAO Ruiguang; JIANG Xueqin; LIU Yongchun; WANG Minglei; XU Yingjiang

doi:10.13984/j.cnki.cn37-1141.2025.04.021

Abstract：With the increase of people's demand for aquatic products,some problems have gradually e-merged,which include unclear information about the origin,confusing the real with the false,shoddy among others.Stable isotopes contain abundant environmental information of origin,and they have a great application prospect in origin traceability.To protect abalone products with geographical indica-tions and trace the origin of abalones,in this study,abalone samples were collected from Lianjiang,Changdao,Rongcheng and Dalian.The stable isotope ratios of carbon and nitrogen in gastropod mus-cle,muscle edge,digestive glands and gonads and mantle of abalones were analyzed on a stable isotope mass spectrometer.Origin traceability model was established through Fisher discriminant analysis,and verified with leave-one-out method.It was pointed out that the fingerprint characteristics of overall sta-ble isotope ratios of abalones from different regions,and the recognition ability of stable carbon and ni-trogen isotopes in different tissues to the origin traceability were evaluated.The results showed that the overall original correct discrimination rate of abalone of origin traceability is 90.5％,the correct dis-crimination rate of cross validation is 89.0％,and Fujian and Rongcheng regions have the best effect on abalone traceability.The correct rate of individual identification each tissue was below 80％,less than the correct rate of identification for all tissues.It was suggested that all organizations should be combined to trace the origin to improve the accuracy of origin traceability.In addition,distinguishing Lianjiang abalones from those of Rongcheng can be achieved using the traceability index of mantle alone.Our findings provided a scientific basis for establishing a new traceability system of aquatic products origin in China.