Studies on the extraction efficiency of Heterocapsa bohainensis toxins based on the lethal effect to Brachionus plicatilis
GONG Xue
SUN Na
FENG Tongtong
ZHANG Yuwei
XUE Tong
ZHANG Yiwen
LIU Lifen
Abstract：Heterocapsa bohaiensis perform a significant toxic effect on Brachionus plicatilis;however,the toxins of H.bohaiensis are not known yet.In addition,whether the algal toxins are stable or not during extraction,separation and identification is crucial.To explore the extraction condition of the toxins of H.bohaiensis,the toxicity of its crude extracts on B.plicatilis was evaluated for a set of ex-traction and preservation conditions in this study.The results indicated that H.bohaiensis toxins are intracellular;a similar rotifer mortality is found in algal cells and ruptured cell suspension but not in cell-free filtrates.Temperature impacted on the algal toxin.It was found that the toxins are most sta-ble at 25℃.Light condition was not concerned with the toxicity of H.bohaiensis.Acetonitrile crude extract from ruptured cell suspension showed the heaviest toxic effect.The maximum absorption wave-length of acetonitrile extraction was 442 nm which is usable in detection during toxin separation.The extraction and preservation conditions of H.bohaiensis toxins were figured out in this study,which will contribute to isolating and structurally identifying the toxins in further.
Keywords：Heterocapsa bohaiensisBrachionus plicatilistoxinextraction conditionharmful algal bloom
Publication Date：2025-06-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 106-112 )