Study on aquiferbioclogging under different dissolved oxygen levels
SUN Jie
YAN Ni
ZHAO Mingmin
ZHENG Xilai
LI Min
Abstract：During the artificial recharge process,the irreversibility of bioclogging influences the service life of the recharge system,and the research on the effect of dissolved oxygen(DO)on bioclogging has scientific significance and application value.In this study,one-dimensional column experiments were used to study the bioclogging process of aquifer under different DO levels.By analyzing the effects of DO on the permeability of the surface of sand column,the structure of microbial community,the num-ber of bacteria and the content of extracellular polymer substances(EPS),had determined the main factors of bioclogging caused by DO in different percolation stages.The results showed that the de-crease rate of permeability of porous media was positively correlated with DO content.When the rela-tive hydraulic conductivity K'of porous media reached 0.2,the recharge time of DO content of 3,6 and 9 mg/L was 21,18 and 16 h,respectively.During the progress of recharge,the bacterial commu-nity structure on the surface of sand column had a significant succession,the abundance of facultative anaerobic bacteria(e.g.,Enterobacteriaceae,Enterobacter and Bacillus)increased,the abundance of aerobic bacteria(e.g.,Acinetobacter)reduced,and the bacterial community diversity was highest in the 9 mg/L DO content test.In addition,during the recharge process,the accumulation of bacterial cells was higher in groups with high DO content,but the secretion of EPS was lower in high DO con-tent.Therefore,DO mainly accelerated the process of bioclogging by affecting the number of bacterial cells.
Keywords：artificial rechargeDOcloggingmicrobesEPS
Publication Date：2025-04-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 59-66 )