Acute toxicity of decabromodiphenyl ether(BDE-209)to and its effect on the digestive enzymes activities of Urechis unicinctus

XU Xinghong; LIU Tonghao; DING Ziyuan; LIU Shun; QI Lu; GAO Yiqiang; ZHOU Chaoyi

doi:10.13984/j.cnki.cn37-1141.2025.01.006

Abstract：To enrich the basic data of the toxic effects of marine environmental hormones on benthic ani-mals,the acute toxicity of decabromodiphenyl ether(BDE-209)with different mass concentrations to and its effect on the digestive enzyme activities of Urechis unicinctus were analyzed in this study.The experimental results showed that LC50 of BDE-209 for U.unicinctus at 48 h,72 h,and 96 h was 153.170 mg/L,96.791 mg/L,and 60.042 mg/L,respectively,while BDE-209 was safe at the concen-tration of 6.004 mg/L.BDE-209 at the concentration of 60 μg/L or less can induce alkaline protease,acid protease,cellulase and lipase significantly,and the maximum induction degree was found at 0.6μg/L.After 3 days of exposure,there was a positive correlation between alkaline protease activity and dose concentration in the treatment group with stress concentration of 0.6 μg/L and below(P＜0.01).Amylase activity was inhibited in all treatments,and a significant negative correlation was observed be-tween amylase activity and concentration after 1 day of exposure(P＜0.05).The activities of digestive enzymes decreased under the long-time stress of BDE-209.After exposure for 15 d,the activities of di-gestive enzymes in all treatments were significantly lower than those in control group except alkaline protease in 60 μg/L or less treatments.Although 6,000 μg/L of BDE-209 was slightly lower than the safe concentration(6.004 mg/L),it showed an inhibited effect on the digestive enzyme activities.BDE-209 was low-moderate toxic to U.unicinctus,and a certain concentration of BDE-209 can induce diges-tive enzymes except amylase,but long-term stress at low concentration or at near-safe concentration can inhibit digestive enzyme activities.