Preliminary Study of Segment Culture of Gracilariabailinae

CHEN Haihong; CHEN Weizhou

Abstract：Different measures of tissue culture of Gracilariabailinae were evaluated in the present study.Four various aspects were taken into consideration including positions of G.bailinae,treatments of sterilization,fragment length and cutting methods.The results showed that the number of budding of trunks was significantly higher than those of middlebranchs and stem tips,indicating that trunks is the best position for tissue culture of G.bailinae.The G.bailinae that was steeped with 0.5g/L penicillin sodium,0.5g/L streptomycinsulphate and 1mg/L GeO2 for 16h and then was immersed into the sterilized seawater about 48h,treated with 70％ ethyl alcohol for 5s or 1.5％KI treated for 10min,had higher survival rates and lower contamination rates of wild seaweed than other treatments.There is no significant difference in budding numbers by using different cutting methods.Among different fragment lengths,2mm fragment is the most economical one that has the biggest bud density.In most of the fragments,budding couldnotn't be found in the lower transection,which showed a significant polarity.