Study on Selective Marker for Genetic Engineering of Saccharina japonica at Different Growth Period
WANG Shanshan
ZHANG Lei
JIN Yuemei
CHI Shan
LIU Cui
LIU Tao
TANG Xuexi
Abstract：To obtain suitable selective markers is a key step for acquiring transgenic kelp.In this study the sensitivity of Saccharina japonica at three development stages including gametophyte,gametophyte clon and young sporophyte was studied under the treatment of three selective reagents including glyphosate (Glp),glufosinate (Glu),and chloramphenicol (Chl).The semi-lethal dose (LD50) of three reagents at certain period was calculated by the SPSS software.The result showed that S.japonica gametophyte and gametophyte clone were sensitive to chloramphenicol,particularly to glyphosate and glufosinate.At the fourth day,the LD50 of gametophyte was 8.19 μg/mL to Glp,19.27 μg/mL to Glu,and the LD50 of gametophyte clone was 73.69 μg/mL to Glp,39.08 μg/mL to Glu.The LD50 of Chl was 701.51 g/mL and 742.00 μg/mL to the gametophyte and gametophyte clone,respectively.S.japonica young sporophyte,however,demonstrated higher sensitity to Glp (40.93 μg/mL) than to Glu (214.51 μg/mL) and Chl (907.86 μg/mL).These results indicated that the glyphosate and glufosinate were suitable selective markers for S.japonica gametophyte and gametophyte clone,while the glyphosate was a suitable selective marker for S.japonica young sporophyte.
Keywords：Saccharina japonicagenetic engineeringselective markersensitivity
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 101-107 )