Preliminary Study on Sample Preparation of Ruditapes philippinarum Haemocyte for Transmission Electron Microscopy
LIU Jing
YUAN Yuqing
WU Yalin
Abstract：The sample preparation directly affects the transmission electron microscopy (TEM) observation and the quality of the electron microscope pictures.Due to the bivalve haemocyte has the characteristics of agglutination,low density and morphological diversity,in order to find the proper haemocyte preparation of bivalve for TEM we compared the effects of sampling method,agar pre-embedded mode and osmium tetroxide fixed time on the haemocyte sample preparation of Ruditapes philippinarum in this paper.The results showed that using anaxenic syringein which contained fixative could effectively prevent haemocytes agglutination.In the agar pre-embedded procedure,it was better to centrifuge the haemocytes after blending the haemocyte into agar and 3000 r/min～10000 r/min rotate speed could achieve good effect of sample preparation.It had significantly effects of osmium tetroxide fixed time on the Ruditapes philippinarum haemocyte ultrastructure,in osmium tetroxide fixed 10 min treatment the cytoplasmic matrix lost seriously,the organelles ultrastructures were not well preserved,with the fixed time increasing,the haemocyte ultrastructures got better,and the osmium tetroxide fixed 2h groups got the best effect of sample preparation for haemocyte.These results provided basic technical reference for studing the haemocyte ultrastructure,related immunology and pathology of R.philippinarum and and other bivalve.
Keywords：Ruditapes philippinarumhaemocytetransmission electron microscopesample preparation
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 113-119 )