Effect of Yuchuang Powder on mucosal damage and inflammatory factors in rats with acetic acid-induced recurrent oral ulcers
GUO Yangzhi
DU Juan
ZHU Ran
PAN Guofeng
YANG Tao
LIU Jiye
QIU Shaochen
Abstract：Objective To investigate the effect of Yuchuang Powder on mucosal tissue damage and the concentrations of tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)in rats with recurrent oral ulcers(ROU)induced by acetic acid.Methods 48 healthy SPF-grade SD rats(equal numbers of males and females)were randomly divided into the blank control group(n=8)and the modeling group(n=40)using a random number table.The modeling group was used to establish the ROU model by acetic acid induction,and then randomly divided into the model group,chitosan group,low-dose Yuchuang Powder group,medium-dose Yuchuang Powder group,and high-dose Yuchuang Powder group,with 8 rats in each group.The chitosan group was treated with oral ulcer gel,while the Yuchuang Powder groups were treated with different doses of Yuchuang Powder.After 7 consecutive days of medication,mucosal tissue pathology was assessed by hematoxylin and eosin(HE)staining,expression levels of TNF-α,IL-6,and IL-1β in tissue were evaluated by immunohistochemistry,concentrations of TNF-α,IL-6,and IL-1β in tissue homogenates were measured by enzyme-linked immunosorbent assay(ELISA),and protein expression levels of TNF-α,IL-6,and IL-1β in ulcer tissue were analyzed using Western blotting.Results HE staining showed that mucosal tissue damage in the medication groups was significantly alleviated compared with the model group Immunohistochemistry indicated reduced expression of TNF-α,IL-6,and IL-1β in the medication groups compared with the model group(all P<0.05).ELISA results showed significantly lower concentrations of TNF-α,IL-6,and IL-1β in the medication groups(all P<0.05).Western blotting confirmed decreased protein expression of TNF-α,IL-6,and IL-1β in the medication groups(all P<0.05).No statistically significant differences were observed among the medication groups(all P>0.05).Conclusion Yuchuang Powder can promote the mucosa lrepair in ROU rats,and this effect may be achieved by reducing the concentrations of TNF-α,IL-6,and IL-1β in ulcer mucosal tissues and alleviating local inflammatory response.
Keywords：recurrent oral ulcersYuchuang Powdertumor necrosis factor-αinterleukin-6interleukin-1β
Publication Date：2026-02-25
Online Publishing Date：2026-03-26(First online date of this platform, not the publication date of the document)
Pages：7( 76-82 )