Tamsulosin mitigates weightlessness induced bone loss by targeting the calcium-activated chloride channel Ano1
WANG Guancheng
LI Yuheng
LI Yingxian
SUN Weijia
Abstract：Objective To investigate the therapeutic effects and mechanisms of the Ano1 calcium-activated chloride channel inhibitor tamsulosin in weightlessness-induced bone loss.Methods With the cell rotation model used to simulate the effects of microgravity,the impact of tamsulosin on the increased activity and function of osteoclasts induced by simulated microgravity was assessed.Using a mouse tail suspension model to simulate microgravity,the effects of tamsulosin on bone loss caused by simulated microgravity were analyzed.Results After simulated microgravity treatment,compared to the DMSO treatment group,the upregulation of osteoclast-related gene expression in the tamsulosin treatment group was reduced,the number of TRAP-positive cells decreased,the expression of Ano1 and its downstream signaling pathway CaMKⅣ-Creb-NFATc1 were inhibited,and intracellular chloride ion concentration increased.Following tail suspension to simulate weightlessness,the bone loss in the tamsulosin-treated mice was reduced.Conclusion tamsulosin targets Ano1 to inhibit osteoclast activity and alleviate weightlessness induced bone loss.
Keywords：calcium-activated chloride channeltamsulosinosteoclastweightlessness induced bone losssimulated microgravity
Publication Date：2025-12-25
Online Publishing Date：2026-01-15(First online date of this platform, not the publication date of the document)
Pages：8( 594-601 )