The effect of microgravity on hibernating myoblasts
Liu Yizhou
Cao Xiaojian
Shi Liujia
Chen Yunqiang
Tan Yingjun
Huang Danxia
Wang Chunyan
Zhou Qiuzhi
Qu Lina
Luo Hongmei
Yin Xuemin
Zhang Song
Liu Zhaoxia
Li Yajie
Xu Jia
Li Yinghui
Chen Hong
Abstract：Objective To investigate the effects of microgravity environment on hibernating myoblasts.Methods Hibernating myoblasts were cultured under real and simulated microgravity conditions for 10 days.RNA-seq analysis and immunofluorescence are used to analysis the impact of microgravity environment on cell growth and gene expression of myoblasts.Results Under the microgravity conditions,genes associated with proliferation were upregulated.Under simulated microgravity,there were more and higher proportion of Ki67 positive cells compared to normal gravity conditions.Conclusion The microgravity environment promotes the proliferation of hibernating myoblasts.
Keywords：hibernationmyoblastsmicrogravityspace flightskeleton muscle regeneration
Publication Date：2024-10-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 275-281 )