Research on Changes of Small Molecule Metabolites in Human under 180-day CELSS Environmental Conditions with High Resolution Mass Spectrometry
Dong Haisheng
Chen Pu
Zhao Wei
Yu Yanbo
Luo Minglei
Han Bingxing
Chen Bin
Abstract：Objective To establish nutritional protection measures scientifically and rationally, by exploring the characteristics of nutritional metabolism in long-term confined environment. Methods Four volunteers were recruited to participate in the 180 d CELSS integration experiment. Morning urine were collected from the four volunteers during the experiment. UHPLC-Q/Exactive high resolution mass spectrometry was used to detect the small molecular metabolites in the urine. The partial least squares discriminant analysis model was used to select the metabolites with significant changes in the confined environment. The effects of the enclosed environment on the nutrient metabolism were studied. Identification of metabolic variation in the confined environment was analyzed by metabolic pathway analysis. Results A total of 70 small molecule metabolites changed significantly, reflecting the impact of confined environment on human protein digestion, energy metabolism, lipid oxidation, intestinal microflora structure and so on. Conclusion The small molecule nutrient metabolites changed during the resident in long-term controlled environment, and it is necessary to adopt nutrition protection measures.
Keywords：Controlled Ecological Life Support Systemhigh resolution mass spectrometrymetabolic markersmetabolic pathway
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 295-300 )