Analysis on Body Microbiota of People Surviving in Controlled Ecological Life Support System of 180 days Experiment

Xin Bingmu; Wang Heng; Wu Yuanliang; Zhong Chongfa; Liu Junlian; Chen Zhanghuang; Dun Huanhuan; Xie Qiong

doi:10.16289/j.cnki.1002-0837.2018.02.027

Abstract：Objective To study body microbiota of people surviving in controlled ecological life support system for 180 days. Methods Each crewmember was sampled at seven different time periods to provide the data: 15 days before entering the isolation facility, then 30 days interval throughout the entire 180-day experiment, and immediately after exiting the module. The body surface sites generally sampled were forehead, restroauricular, chelidon, armpit and inguen. Genomic DNA of the samples was extracted and the 16S rDNA V3-V4 region was amplified by PCR and identified by Illumina. The bioinformatic analysis included the community composition, diversity, principle co-ordinates and species abundance analysis. Results Bacterial diversities of chelidon and forhead were higher than other body surface sites. Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes were dominant on human skin at phylum level. At genus level, corynebacterium and staphylococcus were two of the most prevalent genus. In addition, propionibacterim was the third commonly identified at forehead and retroauticular. Genus-level microbiota of chelidon was different from other sites. The armpit and inguen florae were similar, and forehead and retroauricular were similar. A significant wave on microbial community composition was showed after one month life in confined environment. Conclusion Human skin-associated microorganisms are different in community diversity at specific sites. At genus-level the differences of microbial commmity composition of different body sites are little, but at phylum the differences of which are significant. Confined environment of controlled ecological life support system can be a stress factor to influence microbial homeostasis on body surface.