Effects of Qiweibaizhusan Combined with Yeast on Intestinal Lactobacillus Diversity in Dysbacteriotic Diarrhea Mice
Liu Youjia
Xiao Xinyun
Deng Yanling
Guo Kangxiao
She Yan
Tan Zhoujin
Abstract：Objective To provide microbiological experiment proof for Qiweibaizhusan combined with yeast in clinical application.Methods Antibiotic associated diarrhea mice model was constructed with stomach administration of antibiotic.The antibiotic-associated diarrhea mice were divided into 1 model group and 3 treatment groups.Treatment groups were respectively given 25％ ultra-micro Qiweibaizhusan [0.04 g/(kg · d)] combined with 25％ yeast [0.25 × 1010 yeast/(kg· d)],0.08 g/(kg· d) 50％ amount of ultra-micro Qiweibaizhusan,0.16 g/(kg · d) full amount (100％)of traditional Qiweibaizhusan and 0.16 g/(kg · d) Sterile saline.,Gut microbial metagenome DNA was extracted from the ileum intestinal contents.Then ARDRA analysis was done after PCR by specific primer of Lactobacillus.Results OUTs,Shannon diversity index and Brillouin diversity index were respectively 5,2.3219 and 1.3814 in 25％ amount ultra-micro Qiweibaizhusan combined with 25％ yeast group.All were the same as that in the normal group and 50％ amount of ultra-micro Qiweibaizhusan group,but were more than that in full amount of traditional Qiweibaizhusan group and the model group.The principal component analysis showed that 25％ amount ultra-micro Qiweibaizhusan combined with 25％ yeast group was less than the normal group,but more than 50％ amount of ultra-micro Qiweibaizhusan group,100％ amount of traditional Qiweibaizhusan group and the model group in the cumulative contribution rate.Conclusion 25％ amount ultra-micro Qiweibaizhusan combined with 25％ yeast group is not as good as the normal group.But it is better than 50％ amount of ultra-micro Qiweibaizhusan group,full amount of traditional Qiweibaizhusan group and the model group in adjusting the diversity of Lactobacillus.Qiweibaizhusan combined with yeast group is conducive to treatment of diarrhea.
Keywords：Qiweibaizhusanyeastantibiotic-associated diarrheaintestinal lactobacillus
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)