Interleukin-17A Inhibits Apoptosis Induced by Bleomycin in Mouse NIH/3T3 Cell
Liu Hong
Xin Bingmu
Wang Jiaping
Abstract：Objective To investigate the effect of interleukin-17A treatment on fibroblast cell NIH/3T3 apoptosis induced by bleomycin in mouse.Methods The apoptosis was induced with bleomycin,and detected by TUNEL staining.The expression of cleaved-caspase-3 and Bcl-2 was detected with Western blot.Results The fragmentation of DNA in mouse fibroblast cell NIH/3T3 was promoted with bleomycin treatment.The expression of cleaved-caspase-3 was increased and the expression of Bcl-2 was decreased.The treatment of interleukin-17A inhibited the fragmentaion of DNA in mouse fibroblast cell NIH/3T3 with treatment of bleomycin.The expression of cleaved-caspase-3 was reduced and the expression of Bcl-2 was increased by interleukin-17A treatment.Conclusion Interleukin-17A inhibits apoptosis induced by bleomycin through stimulating Bcl-2 in mouse fibroblast cell.
Keywords：interleukin-17AbleomycinapoptosisNIH/3T3 cell
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)