Diamagnetic Levitation Affects Cellular Morphology and Functions of Osteoblast Adhered to Micro-carrier
Yang Xianyuan
Wang Zhe
Zhang Jian
Hu Lifang
Shang Peng
Abstract：Objective To detect if MC3T3-E1 osteoblast adhered to Cytodex-1 micro-carrier could realize diamagnetic levitation cultivation in large gradient high magnetic field (LGHMF) to simulated weightlessness environment and to determine if MC3T3-E1 osteoblast cell biological responses were affected with this cultivation form under various simulated gravity environments by LGHMF.Methods The murine calvarial osteoblast cell line MC3T3-E1 adhered to Cytodex-1 micro-carrier was subjected to an apparent gravity of μG(12 T),1 G (16 T) and 2 G(12 T) in a LGHMF,respectively.The cell morphology,proliferation,apoptosis and cycle were detected.Results The proliferation of MC3T3-E1 was inhibited in 2 G condition and was promoted in μG and 1 G condition.The LGHMF induced the morphologic change of MC3T3-E1,increased caspase-3 activity and affected the cell cycle.Conclusion MC3T3-E1 adhered to Cytodex-1 micro-carrier could realize diamagnetic levitation and the cells morphology and functions were affected by LGHMF.
Keywords：large gradient high magnetic fielddiamagnetic levitationsimulated weightlessnessmicro-carrier
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)