Dielectric Properties of Rat Whole Blood, Spleen and Testis were Changed by Hypo-magnetic Field and Hind-limb Unloading

Ding Chong; Jia Bin; Liu Xi; Lv Yi; Wang Jia; Shang Peng

Abstract：Objective With dielectric spectrum to evaluate the influences of rats exposed to hypo-magnetic field and simulated microgravity on the dielectric properties of whole blood,spleen and testis.Methods A total of 24 adult male Sprague-Dawley rats were randomly divided into four groups,control (Con) group under geomagnetic field (strength approx.52 μT),hypo-magnetic field (HMF) group under hypo-magnetic conditions (strength approx.50 nT to 230 nT),hind-limb unloading (HLU) group maintained in tail-suspension apparatus,and hypo-magnetic field combined with hind-limb unloading (HMF + HLU) group.After 4-week treatment,whole blood,spleen and testis were taken from all rats.The dielectric spectra were detected with Impedance Analyzer in the frequency range from 0.1 MHz to 120 MHz.The dielectric parameters were estimated with fitting experiment data in the one-pole Cole-Cole model.Results 1) Marked increases in permittivity up to 19.6％ and in conductivity up to 19.1％ were obtained in HMF group for whole blood.The same results were found in HMF + HLU group ; 2) Conductivity of spleen slightly increased after HLU treated ; 3) Permittivity decreased to 21.9％,but conductivity increased to 49.3％ in HLU group for testis; 4)The dielectric parameters fitted with experiment data in treated groups were the same trend with the changes of dielectric properties.Conclusion Dielectric properties of whole blood,spleen and testis affected by HMF and HLU can be evaluated with dielectric spectroscopy and the dielectric properties of tissue in treated group were different from those in Con group,and the changes varied with different tissues.