Clinostat Induced Microgravity Activates Autophagy in Neuronal Cells
Qiao Wenyan
Sun Qiao
Liang Fengji
Lv Ke
Liu Hongyu
Xiong Jianghui
Li Yinghui
Abstract：Objective To explore whether autophagy are involved in the effects of microgravity on the neuronal cells,we investigate the expression of autophagy-related gene LC3 in SH-SY5Y cells under simulated microgravity.Methods Microgravity was simulated by clinostat.After 12 h and 24 h clinorotation,GFP-LC3 puncta were examined by a fluorescence microscope,and then the number of GFP-LC3 puncta ( ＞ 5 puncta per cell) were counted.The expression levels of LC3-Ⅰ,LC3-Ⅱ protein were detected by Western Blotting.Results After clinorotation,the number of GFP-LC3 puncta increased significantly compared with group control (P ＜ 0.05).Moreover,LC3 protein expression also showed a significant accumulation.Conclusion Simulated microgravity with clinostat can increase autophagosomes in SH-SY5Y cells.
Keywords：simulated microgravityclinorotationautophagySH-SY5YLC3 protein
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)