Activated Caspase-8 Regulates Bcl-2 Expression in Fas- and Actinomycin D-induced Apoptosis in Bel-7402 Cells
WANG Lu
WANG Yu
XIA Chun-hui
Abstract：Objective To investigate whether caspase-8 regulates Bcl-2 expression in Fas and Actinomycin D (AD) induced apoptosis in Bel-7402 cells. Methods Bel-7402 cells were divided into four groups randomly, that is: control, Fas group, Fas-AD group and Ac-IEFD-cho group, and treated with Fas-AD in the presence/ absence of caspase-8 inhibitor Ac-IEFD-cho. The expressions of caspase-8 and Bcl-2 were determined with Western Blot and RT-PCR, respectively. Results Compared with control group and Fas group, after Bel-7402 cells were treated with Fas-AD, typical apoptosis morphology was clearly observed, the expression of activated caspase-8 increased significantly, and the Bcl-2 expression decreased apparently. However, after Bel-7402 cells were treated with Fas-AD in the presence of the caspase-8 inhibitor Ac-IEFD-cho, the expressions of activated caspase-8 decreased while the Bcl-2 expression increased. Conclusion Activated caspase-8 can regulate Bcl-2 in Fas- and AD- induced apoptosis in Bel-7402 cells.
Keywords：Fascaspase-8Bcl-2cell apoptosis
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 31-34 )