Influences of Hyper-gravity on Early Development, Otolith Morphology and Otolith Chemical Compositions of Zebrafish Larva, Danio Rerio
LI Xiao-yan
WANG Gao-hong
LIU Yong-ding
Abstract：Objective To study zebrafish, as model vertebrate, on its early development and otolith changes under hypergravity, trying to disclose the adaptation mechanism of vestibular system of vertebrate animal to hypergravity. Methods Zabrafish zygotes were put into 1 G and 5 G instruments respectively. Their development status and otolith morphology were observed. Otolith size was measured with the software of Zeiss. Otolith trace elements were examined with EDAX. Results 1 ) Hypergravity influenced early development of zebrafish from zygote to early larva, and otolith size significantly larger in the early stage (P <0.01 ) but decreased subsequently. 2) Examined with EDAX, the contents of sulfur and Sr increased while Ca and Na contents decreased. 3 ) It was observed that sagitta and iapilli otoliths were fused into one otolith. Conclusion Hypergravity influences early development of zebrafish and causes changes of otolith size, morphology and microchemistry, resulting in the change of otolith function.
Keywords：hypergravityotolithmorphologymicrochemistryzebrafish
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 399-403 )