Establishment and Verification of Reference Ranges for 25-OH Vitamin D in Pregnant Women in Zhengzhou,Henan Province
MENG Xiangying
LI Hongwei
LI Hao
WANG Zhuyuan
ZHANG Shuo
YUAN Enwu
ZHANG Linlin
Abstract：Objective To establish the reference interval for 25-OH vitamin D[25(OH)D]in pregnant women in Zhengzhou,Henan Province.Methods This study collected serum samples from 9 411 healthy pregnant women aged from 20 to 56 years who underwent prenatal checkups in Zhengzhou.The 25(OH)D levels were measured using the Siemens ADVIA Centaur XP chemiluminescence immunoassay analyzer.The reference intervals were determined by analyzing the test results and establishing percentiles(P2.5,P97.5).Subsequently,serum samples from an additional 2 664 healthy pregnant women undergoing prenatal checkups in Zhengzhou were collected,and the previously established reference intervals were validated according to the standard WS/T 402-2024 Determination of Reference Intervals of Quantitative Test Items in Clinical Laboratory.Results The reference interval of 25(OH)D in healthy pregnant women aged 20-56 years needed to be grouped by gestational week,season,and age.The reference intervals were as follows:early pregnancy was 12.45-64.00 nmol·L-1,mid-pregnancy was 12.33-63.77 nmol·L-1,late pregnancy was 12.89-77.67 nmol·L-1;spring was 12.67-67.68 nmol·L-1,summer was 13.95-67.11 nmol·L-1,autumn was 13.98-71.23 nmol·L-1,winter was 11.56-64.14 nmol·L-1;age-appropriate pregnancy was 12.45-67.18 nmol·L-1,advanced maternal age pregnancy was 12.47-69.88 nmol·L-1.Results from 2 664 validation specimens showed that＞90％fell within the reference range.Conclusion This study has preliminarily established the reference interval of 25(OH)D in healthy pregnant women aged 20-56 years in Zhengzhou,which has been verified and applied to clinical practice.This study is of key significance for the design of randomized controlled studies to analyze the effects of vitamin D(VD)supplementation on pregnancy outcomes and perinatal infants in different populations.By studying the correlation between VD supplementation and pregnancy outcomes,the optimal serum VD concentration for supplementation in Zhengzhou area can be determined.
Keywords：pregnant women25-OH vitamin Dreference interval
Publication Date：2026-07-13
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 2310-2314 )