Mechanistic Investigation of Alginate Oligosaccharides Alleviating ox-LDL-Induced Endothelial Cell Injury by Intervening in HMGB1-Regulated GSK-3β Signaling Pathway
WEI Xinwei
WANG Zhibin
TIAN Wei
LIU Jianya
Abstract：Objective To investigate the impact of alginate oligosaccharides(AOS)on atherosclerosis(AS)and its underlying potential mechanisms.Methods Human umbilical vein endothelial cells(HUVECs)were used as the research subjects.Cell viability was assessed using the CCK-8 assay.Gene expression was detected by qRT-PCR and Western blot.Cell apoptosis and reactive oxygen species levels were evaluated through flow cytometry.Superoxide dismutase(SOD)activity was measured using an SOD kit.The contents of inflammatory factors interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were determined by ELISA.Results AOS effectively alleviated the inhibitory effect of oxidized low-density lipoprotein(ox-LDL)on HUVECs viability and partially reversed the promoting effect of ox-LDL on high mobility group box 1 protein(HMGB1)expression.The administration of AOS significantly attenuated the ox-LDL-induced suppression of HUVECs viability,promotion of apoptosis,and exacerbation of oxidative stress and inflammatory response.However,HMGB1 overexpression partially counteracted these beneficial effects of AOS.In ox-LDL-induced HUVECs,AOS successfully activated the glycogen synthase kinase-3β(GSK-3β)pathway by inhibiting HMGB1 expression.Conclusion AOS demonstrated potential therapeutic effects on AS,and its mechanism might be related to the regulation of HMGB1 expression and activation of the GSK-3β pathway,providing a new perspective for AS treatment.
Keywords：alginate oligosaccharideshigh mobility group box 1 proteinglycogen synthase kinase-3βatherosclerosis
Publication Date：2026-04-28
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：8( 1375-1382 )