Comparative of Carbapenemase Inhibitor Enhancement Test and Colloidal Gold Immunochromatography Assay in Detection of Carbapenemase from Enterobacteriaceae
WANG Xiaokun
XIAO Weiqiang
QU Yuanye
SUN Mingyue
CHANG Yanmin
XU Qingxia
Abstract：Objective To evaluate the application of carbapenem inhibitor enhancement test and NG-Test CARBA5 colloidal gold immunochromatographic assay in the detection of carbapenemase of Enterobacteriaceae.Methods Non repetitive carbapenem-resistant enterobacterales(CRE)isolated from patients were collected at the Affiliated Cancer Hospital of Zhengzhou University in 2021.The enzyme type and gene type were detected by carbapenem inhibitor enhancement test and NG-Test CARBA5 colloidal gold immunochromatography assay respectively.The results of polymerase chain reaction(PCR)was set as the standard.Results Compared with the results of PCR,carbapenemase inhibitor enhancement test detected class A carbapenemase or class B metallo-beta-lactamase,the sensitivity and specificity were 100.00％and 98.36％,respectively.Colloidal gold immunochromatographic assay detected class A carbapenemase or class B metallo-beta-lactamase,the sensitivity and specificity were 95.90％and 99.18％,respectively.Conclusion The carbapenemase inhibitor enhancement test and NG-Test CARBA5 colloidal gold immunochromatographic assay have high sensitivity and specificity in detecting the carbapenemase type and genotype of Enterobacteriaceae bacteria.
Keywords：carbapenemase inhibitor enhancement testcolloidal gold immunochromatographycarbapenem-resistant enterobacteralesNG-Test CARBA5
Publication Date：2026-03-28
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 971-975 )