Expression of ACP1 in Hepatocellular Carcinoma and Its Effect on Metastatic Activity of Huh7 Cells
SONG Huayong
ZHANG Fan
LI Xiaoyun
CHENG Yanhui
Abstract：Objective To investigate the expression of acid phosphatase 1(ACP1)in hepatocellular carcinoma(HCC)and its effect on the metastatic activity of Huh7 cells.Methods The expression of ACP1 in HCC tissues and adjacent tissues was detected by immunohistochemistry and Western blotting.Huh7 cells were divided into three experimental groups:negative control group,siACP1 group and inhibitor group.The proliferation activity of Huh7 cells was analyzed by CCK-8 assay.The apoptosis rate of Huh7 cells was analyzed by in situ end labeling.Transwell assay was used to analyze the invasion and migration of Huh7 cells.Western blotting was used to detect the expression levels of ACP1 and Wnt pathway-related proteins in Huh7 cells.Results The expression level of ACP1 in HCC tissues was higher than that in adjacent tissues(P＜0.05).Compared with the negative control group,the proliferation ability,invasion and migration ability of Huh7 cells in the siACP1 group and the inhibitor group were decreased,and the apoptosis rate was increased(P＜0.05).Compared with the negative control group,the expression of ACP1,Wnt3α and β-catenin in Huh7 cells in the siACP1 group and the inhibitor group was decreased(P＜0.05),while the expression level of ACP1 in Huh7 cells in the inhibitor group did not change significantly(P＞0.05).Conclusion The expression of ACP1 is increased in HCC tissues.Down-regulation of ACP1 expression can inhibit the proliferation,invasion and migration of Huh7 cells,and increase the apoptosis rate of Huh7 cells.This process is related to ACP1 regulation of Wnt/β-catenin.
Keywords：hepatocellular carcinomaacid phosphatase 1Wnt3αapoptosisHuh7proliferationmigrationinvasion
Publication Date：2025-10-13
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 3499-3503 )