Effect and Mechanism of Silencing MRPS18A on Proliferation,Apoptosis,Migration and Invasion of Cholangiocarcinoma Cells
LIU Xinhui
HU Shoukui
GE Jiejie
LÜ Pengju
Abstract：Objective To investigate the effect of silencing mitochondrial ribosomal protein S18a(MRPS18A)on proliferation,apoptosis,migration and invasion of cholangiocarcinoma cells and the related mechanism.Methods The relative expression difference of MRPS18A in cholangiocarcinoma tissues and normal bile duct tissues was analyzed by GEPIA2 database,and the survival difference between patients with high expression of MRPS18A and patients with low expression of MRPS18A was analyzed.The expression of MRPS18A in bile duct cancer cells and normal bile duct epithelial cells was detected.The expression of MRPS18A in cholangiocarcinoma cells RBE and QBC939 was silenced by cell transfection.RBE and QBC939 cells were randomly divided into si-NC group and si-MRPS18A-1 group.The proliferation levels of RBE and QBC939 cells were detected by MTT and clonal formation assay.The apoptotic levels of RBE and QBC939 cells were detected by flow cytometry and TdT-mediated dUTP nick and labeling(TUNEL)staining.The migration and invasion levels of RBE and QBC939 cells were detected by Transwell.MRPS18A,p21,Cyclin B1,B-cell lymphoma-2-associated X protein(Bax),B-cell lymphoma-2(Bcl-2),cleaved cysteine aspartate protease-3(cleaved Caspase-3),E-cadherin(E-cad)and N-cadherin(N-cad)were detected by Western blot.Results The expression level of MRPS18A in cholangiocarcinoma tissues was higher than that in normal bile duct tissues(P＜0.05).The overall survival and disease-free survival of patients with high expression of MRPS18A were lower than those with low expression of MRPS18A(P＜0.05).The expression levels of MRPS18A in RBE,QBC939,HuH28 and KMBC in biliary cholangiocarcinoma cells were higher than those in normal biliary duct epithelial cells(P＜0.05).Compared with si-NC group,The levels of MRPS18A,OD450,clonogenesis number,cell migration number,cell invasion number,Cyclin B1,Bcl-2 and N-cad levels of RBE and QBC939 cells in si-MRPS18A-1 group were decreased(P＜0.05).While the apoptosis rate,apoptosis index,p21,Bax,cleaved Caspase-3 and E-cad levels were increased(P＜0.05).Conclusion Silencing MRPS18A can inhibit the proliferation,migration and invasion of cholangiocarcinoma cells and induce cell apoptosis.The mechanism may be related to the regulation of cell cycle,mitochondrial apoptosis pathway and epithelial-mesenchymal transition process by MRPS18A.
Keywords：cholangiocarcinomaMRPS18Aproliferationapoptosismigrationinvasion
Publication Date：2025-04-28
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：8( 1391-1398 )