Effects of Danyu Tongmai Granules on Activation,Proliferation and Migration of Cardiac Fibroblasts Induced by Angiotensin Ⅱ

MAO Liwei; WU Guanghua

doi:10.3969/j.issn.1004-437X.2025.08.008

Abstract：Objective To study the effects of Danyu Tongmai Granules on activation,proliferation and migration of cardiac fibroblasts induced by angiotensin Ⅱ.Methods The isolated neonatal rat myocardial fibroblasts were divided into 5 groups,namely control group,model group,low dose Danyu Tongmai Granules(0.5 g·L-1)group,medium dose Danyu Tongmai Granules(1 g·L-1)group and high dose Danyu Tongmai Granules(2 g·L-1)group.Control group was given normal medium,model group was given 1 μmol angiotensin Ⅱ,and drug group was given different concentrations of Danyu Tongmai Granules to pretreat myocardial fibroblasts for 2 hours,then incubated with 1 μmol angiotensin Ⅱ for 24 hours.MTT method was detected proliferation of myocardial fibroblasts.Migration was detected by scratch and Transwell method.Western blot method was used to detect the expression of myocardial fibroblast activation and collagen protein.Results Compared with control group,myocardial fibroblasts and collagen expression in model group increased(P＜0.05),indicating that the in vitro myocardial fibrosis model was successfully constructed.Compared with model group,survival and migration rate of cell in drug group were significantly decreased(P＜0.05),and collagen expression was decreased(P＜0.05),Danyu Tongmai Granules concentration increased,inhibitory effect was more obvious.Conclusion Danyu Tongmai Granules can effectively inhibit activation,proliferation and migration of neonatal rat myocardial fibroblasts induced by angiotensin Ⅱ,and reduce the expression of collagen protein,and the inhibitory effect shows a concentration-dependent trend,the mechanism may be related with the inflammatory pathway(NF-κB).