Mechanism of Isoxanthohumol Inhibiting Cisplatin-Induced Apoptosis and Migration of Lung Cancer A549 Cells

WANG Xiaohui; WU Naipu; ZANG Dan; XUE Yun; CHEN Yiyang; CUI Yuanyuan; DI Wenyu

doi:10.3969/j.issn.1004-437X.2025.08.007

Abstract：Objective To explore the mechanism by which isohumol(IN)inhibits cisplatin(DDP)-induced proliferation inhibition,apoptosis,and migration of lung cancer A549 cells.Methods Human lung cancer cell line A549 was cultured in vitro and randomly divided into control group,DDP group,and DDP+IN group.The control group A549 cells were untreated,the DDP group A549 cells were treated with 6 mg·L-1 DDP for 48 hours,and the DDP+IN group A549 cells were treated with 6 mg·L-1 DDP and 10 μ mol·L-1 IN for 48 hours.A549 cell activity was detected by CCK-8 method,cell apoptosis was detected by flow cytometry,cell invasion and migration ability was detected by transwell chamber,and protein expression was detected by Western blotting.Results Compared with the control group,the DDP group and DDP+IN group showed a decrease in cell viability,invasion,and migration of A549 cells(P＜0.05),and the DDP+IN group had higher cell viability and more invasion and migration cells than the DDP group(P＜0.05).Compared with the control group,the apoptosis rate of A549 cells increased in the DDP group and DDP+IN group(P＜0.05),and the apoptosis rate of A549 cells in the DDP+IN group was higher than that in the DDP group(P＜0.05).Bcl 2-associated X protein(Bax),Bax/B cell lymphoma/leukemia-2 protein(Bcl 2),and Caspase-3 and E-cadherin were higher in A549 cells of the DDP+IN group(P＜0.05),while Bcl 2,Vimentin,N-cadherin,and Snail were significantly higher in A549 cells than in the DDP group(P＜0.05).Conclusion IN can increase DDP induced apoptosis and invasion/migration inhibition in A549 cells,and its mechanism is related to IN regulating the expression of apoptosis related proteins and epithelial mesenchymal transition related proteins.