Clinical Significance of Changes in Expression of miRNA-23 a and miRNA-146a in Gingival Crevicular Fluid Before and After Periodontal Treatment

LU Yuping; WANG Qianqian

doi:10.3969/j.issn.1004-437X.2025.07.018

Abstract：Objective To explore the clinical significance of changes in expression of miRNA-23a and miRNA-146a in gingival crevicular fluid before and after periodontal treatment.Methods From October 2019 to April 2022,170 patients with periodontitis treated in Zhengzhou Yihe Hospital and 68 healthy medical examiners during the same period were selected as the research subjects,and were used as the study group and the control group,respectively.According to the degree of periodontitis in the study group,the patients were divided into mild,moderate and severe patients,65,58 and 47 patients.The expression levels of interleukin(IL)-1β,IL-6,IL-10,tumor necrosis factor-α(TNF-α),IL-4 and interferon-γ(INF-γ)in gingival crevicular fluid were detected by the enzyme-linked immunosorbent assay before and after treatment for 6 weeks.The expression levels of miRNA-23a and miRNA-146a in the gingival crevicular fluid were detected by real-time fluorescence quantitative PCR before and after treatment for 6 weeks.The relationship between the expression levels of miRNA-23a and miRNA-146a and the inflammatory factors and clinical indicators of patients with periodontitis were also analyzed.Results The expression levels of miRNA-23a,miRNA-146a and proinflammatory cytokine(IL-1β,IL-6,TNF-α)in the gingival crevicular fluid of the study group were higher than those of the control group(P＜0.001).The expression levels of anti-inflammatory cytokine IL-10 and IL-4/INF-γ of the study group were lower than those of the control group(P＜0.05),and there was a statistically significant difference between the above factors in the three groups of mild,moderate and severe(P＜0.05).After treatment,miRNA-23a,miRNA-146a,and clinical indicators(AL,PD,BOP positive rate)all decreased significantly compared with those before treatment(P＜0.05).Pearson correlation analysis showed that the expression levels of miRNA-23a and miRNA-146a in gingival crevicular fluid were significantly positively correlated with the expression levels of inflammatory factors IL-1β,IL-6 and TNF-α,AL,PD,and there was a significant negative correlation with IL-10 expression level(P＜0.05).Conclusion The expression of miRNA-23a and miRNA-146a in gingival crevicular fluid of patients with periodontitis was significantly high,and there was a significant correlation with the expression of inflammatory factors.And periodontal basic therapy can improve periodontitis patients'inflammatory response by reducing miR-23a and miRNA-146a.