Thin-Layer Chromatography Identification and the Content Determination of the Total Flavonoids in Scorzonera Albicaulis Bunge
LIU Dong
YANG Qingyu
HUA Zhihui
ZHANG Sisen
WU Guoxue
WANG Xiaoyan
SHEN Dexin
Abstract：Objective To establish the quality control standard of Scorzonera albicaulis Bunge.Methods A thin-layer chromatography method was used to identify rutin in Scorzonera albicaulis Bunge.With nitrite-aluminum nitrate-sodium hydroxide as the chromogenic reagent,the content of the total flavonoids in Scorzonera albicaulis Bunge was detected by ultraviolet-visible spectrophotometry at 508 nm.Results The result of thin-layer chromatography identification showed that there was a spot of the same color in the chromatographic position corresponding to the chromatographic position of rutin control.The content determination result showed that there was a better linear relationship between concentration of rutin and absorbance in the range of 7.86-47.18 mg·L-1.The linear regression equation was A=0.013 2C-0.002 1(r=0.999 9),in which the C was the mass concentration of rutin reference(mg·L-1),A was the absorbance.The average recovery of rutin was 99.12％(RSD=1.53％).Conclusion The research method is simple,accurate and repeatable,which can be used for the quality control standard of Scorzonera albicaulis Bunge.
Keywords：Scorzonera albicaulis Bungerutintotal flavonoidsthin-layer chromatographycontent determinationultraviolet-visible spectrophotometry
Publication Date：2025-03-13
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 769-772 )