Effect and Mechanism of miRNA-299-3p on the Proliferation,Apoptosis,and Migration of Gastric Cancer AGS Cells

FAN Jinyang; DU Botao; CHENG Zhitong

doi:10.3969/j.issn.1004-437X.2025.02.002

Abstract：Objective To explore the effect of miRNA-299-3p on proliferation,apoptosis,cell cycle and migration of gastric cancer AGS cells,and to further explore its mechanism.Methods Gastric cancer AGS cells in vitro were culture and divided into blank control group,negative control group and experimental group.The expression of miRNA-299-3p were detected by qPCR,and transfected the empty vector plasmid and miRNA-299-3p plasmid into AGS cells.The CCK-8 was used to detect cell proliferation,and used flow cytometry to detect apoptosis,cell cycle and reactive oxygen levels.The Transwell was used to detect cell migration and invasion.The Western blot was used to detect changes in related protein expression.Results The CCK-8 experiment showed that high expression of miRNA-299-3p inhibited AGS cell proliferation(P＜0.05).Flow cytometry and Western blot results showed that high expression of miRNA-299-3p can induce AGS cell apoptosis,caspase-3 and Bax expression increased,Bel-2 protein expression decreased(P＜0.05).Meanwhile,after overexpression of miRNA-299-3p,it was able to down-regulate the expression of CDK1/2 and Cyclin B1 protein to block AGS cell cycle in G2/M phase.Transwell chambers and Western blot experiments showed that overexpression of miRNA-299-3p reduced the expression of N-cadherin protein and increased the expression of E-cadherin protein to inhibit the migration of AGS cells.In addition,the miRNA-299-3p overexpression could be up-regulated reactive oxygen levels in AGS cells.Conclusion Overexpression of miRNA-299-3 can inhibit the proliferation of AGS cells,induce apoptosis of AGS cells,block the cell cycle at G2/M phase,and inhibit migration by up-regulating the level of intracellular reactive oxygen species,and this can provide a certain theoretical basis in gastric cancer research for miRNA-299-3p.