Expression of LDOC1 and GNL3L in Gastric Cancer Tissue and Their Relationship with Pathological Characteristics and Prognosis

MAO Jianna; SHEN Yuhou; DUAN Hualing; GAO Qi

doi:10.3969/j.issn.1004-437X.2024.21.016

Abstract：Objective To investigate the relationship between pathological features and prognosis and the expression levels of guanine nucleotide-binding protein-like 3-like protein(GNL3L)and leucine zipper down-regulating factor-1(LDOC1)in the cancer tissues of patients with gastric cancer.Methods Sixty-five cases with gastric cancer admitted to Xinxiang Central Hospital from December 2019 to December 2022 were selected as the study subjects,and the cancer tissue samples(gastric cancer group)and corresponding paracancerous tissue samples(paracancerous group)of gastric cancer patients were collected.Using immunohistochemistry,the expression levels of GNL3L and LDOC1 in tissues were determined.The relationship between the LDOC1,GNL3L and the clinicopathological characteristics of gastric cancer cases were explored byx2 test,and the relevant factors affecting the prognosis of gastric cancer cases explored by multivariate Cox regression.Results The positive rate of LDOC1 in gastric cancer group(30.76％)was lower than that in adjacent cancer group(61.52％),and the positive rate of GNL3L(72.31％)was higher than that in adjacent cancer group(43.07％)(x2=12.381,11.377,P＜0.05).The negative rate of LDOC1 and positive rate of GNL3L were higher in patients with low differentiation,tumor diameter＞3 cm,TNM stage Ⅲ/Ⅳ and lymph node metastasis than those in cases with medium/highly differentiation,tumor straight ≤3 cm,TNM stage Ⅰ/Ⅱ and no lymph node metastasis(P＜0.05).The 3-year survival rate of cases with positive expression of LDOC1(85.00％)was higher than that of cases with negative expression of LDOC1(37.77％),and the 3-year survival rate of cases with positive expression of GNL3L(36.17％)was lower than that of cases with negative expression of GNL3L(94.44％)(P＜0.05).The 3-year survival rate of cases with poorly differentiated,TNM stage Ⅲ/Ⅳ and lymph node metastasis was lower than that of cases with moderately/highly differentiated,TNM stage Ⅰ/Ⅱ and no lymph node metastasis(P＜0.05).Multivariate Cox regression analysis showed that TNM stage Ⅲ-Ⅳ(HR=2.113,95％CI:1.425-3.133),lymph node metastasis(HR=2.625,95％CI:1.564-4.404),LDOC1 negative expression(HR=5.607,95％CI:3.408-9.224)and GNL3L positive expression(HR=2.930,95％CI:1.716-5.003)were prognostic risk factors for patients with gastric cancer(P＜0.05).Conclusion Patients with gastric cancer have low expression of LDOC1 and high expression of GNL3L,and they are associated with the clinicopathological features and prognosis of patients.