Application of Nucleic Acid Testing and Serological Testing in the Diagnosis of Mycoplasma Pneumoniae Infection
TAO Zhanling
SUN Hongqi
YANG Junmei
ZHANG Zheying
Abstract：Objective To compare the nucleic acid testing and three serological detection methods in the diagnosis of Mycoplasma pneumoniae infection.Methods Throat swabs and serum samples were collected from children hospitalized with respiratory infections from January to December 2018 in the Children's Hospital Affiliated to Zhengzhou University,throat swabs was used for Mycoplasma pneumoniae nucleic acid testing,and magnetic particle chemiluminescence,enzyme-linked immunosorbent assay and passive agglutination were used to detect Mycoplasma pneumoniae IgM antibodies(serum samples).The samples with inconsistent nucleic acid and IgM antibody test results were further analyzed by antibody dynamic monitoring.Results There were 96 positive cases and 112 negative cases in nucleic acid detection of Mycoplasma pneumoniae.The consistency between nucleic acid and the three serological methods was medium(Kappa value was 0.457 to 0.509),while the consistency among the three serological methods was high(Kappa value was 0.841 to 0.932).31.25％(30/96)of the acutely infected patients were nucleic acid positive but IgM negative on admission,however,based on the analysis of dynamic changes of IgG antibodies,11.46％(11/96)of the patients could be excluded from acute infection,possibly colonization after infection,10.71％(12/112)of the patients may have missed nucleic acid test results due to inaccurate sample collection.Conclusion The detection of nucleic acid and IgM antibody has its advantages and disadvantages,which should be considered in combination with clinical diagnosis and onset time.
Keywords：Mycoplasma pneumoniaeserologynucleic acidantibody
Publication Date：2023-12-31
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 4433-4436 )