Based TGF-β1/ERK1/2 Signal Pathway Study on the Malignant Biological Activity and Autophagy of TM4SF1 in Nasopharyngeal Carcinoma Cells

LI Zengpei; LI Jing

doi:10.3969/j.issn.1004-437X.2023.23.010

Abstract：Objective To investigate the effects and mechanisms of transmembrane 4 L6 family member 1(TM4SF1)on the proliferation,invasion,apoptosis and autophagy of nasopharyngeal carcinoma cells.Methods CNE-2 cells were divided into si-TM4SF1 group,si-NC group,LY3200882 group,TGF-β1 group and Control group.MTT method was used to detect cell proliferation ability.Transwell method was used to detect cell invasion.Flow cytometry was used to detect apoptosis.Monodansulfonyl cadaveramide(MDC)was used to detect autophagy.TGF-β1,ERK1/2,p-ERK1/2,LC3Ⅱ,LC3Ι,p62 and Beclin1 protein expressions were detected by western blot detection cells.Results Compared with the Control group,TM4 SF1 mRNA expression,proliferation and invasion ability were reduced in the si-TM4SF1 group and LY3200882 group cells,and apoptosis rate and autophagic vesicle fluorescence intensity were increased(P＜0.05).Compared with the si-TM4SF1 group,TM4 SF1 mRNA expression,proliferation,and invasion ability were increased,and apoptosis rate and autophagic vesicle fluorescence intensity were decreased in TGF-β1 group cells(P＜0.05).Compared with the Control group,TGF-β1,p62 protein and p-ERK1/2/ERK1/2 ratio were decreased and Beclin1 protein and LC3Ⅱ/LC3Ιratio were increased in the cells of si-TM4SF1 and LY3200882 groups(P＜0.05).Compared with the si-TM4SF1 group,the TGF-β1 group cells TGF-β1,p62 protein and p-ERK1/2/ERK1/2 ratio were increased,and Beclin1 protein and LC3Ⅱ/LC3Ιratio were decreased in the TGF-β1 group(P＜0.05).Conclusion Low expression of TM4SF1 can inhibite the proliferation and invasion of nasopharyngeal carcinoma cells and promote apoptosis and autophagy,and its mechanism of action may be related to the inhibition of TGF-β1/ERK1/2.