Effects of lappaconitine on IL-1β, IL-10 and TNF-α levels in rats with spinal cord injury
Huang Weiyan
Hou Mingyong
Ou Shan
Abstract：Objective To observe the effect of lappaconitine (LA) on the water content of spinal cord and the spinal cord levels of IL-1β, IL-10 and TNF-α in rats with spinal cord injury (SCI) as well as its neuroprotective function.Methods A total of 240 male Sprague-Dawley rats were randomly divided into SCI group, SCI + saline group (saline group), SCI + LA group (LA group) and SCI + Methylprednisolone group (MP group).With 60 rats in each group, each group contained time points 1, 3, 6, 12, 24 h.The rats in the LA group were injected intraperitoneally immediately with LA (4 mg/kg);the rats in the saline group received intraperitoneal injection of normal saline, 4 mg/kg;the rats in the MP group were injected intraperitoneally immediately with the first dose of 30 mg/kg MP, then were administered according to 5.4 mg/ (kg·h), total amount of 23 h, divided into four injections.The water content of spinal cord and the levels of IL-1β, IL-10 and TNF-α in the spinal cord were measured at each time point.Results Spinal cord water content was significantly increased after SCI.The water content of the spinal cord in LA group and MP group was significantly lower than that in SCI group at the 24 h after SC injury (P<0.01).After SCI injury, the IL-1β, IL-10 and TNF-α levels in the spinal cord increased significantly in each group.At 3, 6, 12, 24 h after SCI, the TNF-α levels of the spinal cord in LA group and MP group were significantly lower than SCI group (P<0.05).At 3, 6, 12, 24 h after SCI, the IL-10 levels in the spinal cord in LA group and MP group were significantly higher than SCI group (P<0.05).The IL-1β levels of spinal cord in the LA group were significantly lower than SCI group at each time point after operation (P<0.05).The IL-1β levels of spinal cord in the MP group were significantly lower than that in SCI group at 6, 12, 24 h after operation (P<0.05).Conclusion LA and MP can attenuate the water content of spinal cord after SCI injury, decrease the levels of IL-1β and TNF-α in spinal cord and improve the levels of IL-10 of the spinal cord, so as to play a neuroprotective role in SCI rats.
Keywords：spinal cord injurylappaconitinewater contentcytokines
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 1729-1732 )