Bacterial culture and drug sensitivity analysis of prostate fluid samples

Zhang Weixing; Chang Keyi; Wang Rui

doi:10.3969/j.issn.1004-437X.2017.03.003

Abstract：Objective To analyze the pathogenic bacteria distribution and drug resistance of chronic prostatitis,offer a better way for the diagnosis and treatment of chronic prostatitis.Methods Patients with prostatitis were asked to stop the treatment of any antibiotics for more than 3 days,clean their orificium urethrae externum prior to collecting prostatic fluid.Midstream urine and prostatic fluid samples were collected for bacterial culture and drug sensitivity test, and the blood samples of 30 patient were taken for detecting procalcitonin.Results In all 121 prostatic fluid samples, 41 of them showed positive result in bacterial culture, the positive rate was 33.88%;43 bacterial strains, including 40 strains of Gram-positive bacteria, accounting for 93.02%, 3 strains of Gram-negative bacteria, accounting for 6.98%.Drug sensitivity results from 38 cases of staphylococcal strains showed they were all sensitive to quinupristin-dalfopristin, linezolid, vancomycin and tigecycline, but resistant to penicilinc, erythromycin and other antibiotics.Drug sensitivity results from 3 strains of Gram-negative bacteria showed they were sensitive to common antibiotics.In all 30 blood samples for detecting procalcitonin, 27 of them showed positive results (PCT>0.02 ng/ml), accounting for 90.00%.The NIH-CPSI between pre-treatment and post-treatment were (15.4±4.3) and (4.2±3.6) (P<0.001).Conclusion Gram-positive cocci occupy a large proportion in the pathogenic bacteria distribution of chronic prostatitis.Detecting procalcitonin may have some significance for the diagnosis of patients with chronic bacterial prostatitis.Doctors should pay attention to the value of bacterial culture and antibiotics in the diagnosis and treatment of patients with chronic prostatitis, and strictly comply with the using indication of antibiotics to prevent abuse of antibiotics.