Regulatory effects of ESCCAL-1 on protein kinase phosphorylation in esophageal squamous cell carcinoma
Cao Xinguang
Cui Yuanbo
Chen Xiaobing
Cao Wei
Abstract：Objective To explore the possible mechanism of ESCCAL 1 in esophageal squamous cell carcinoma. Methods Human phospho - kinase array kit was used to detect the phosphorylation of protein kinase in EC9706 cells transfected with ESC-CAL 1 - shRNA or control. Each gray value of protein band was quantitatively analyzed. Results The relative gray value ratios of FAK、pJNK1、pSrc、pAMPKα1 between KD group and NC group were 2. 76:1,2. 20:1,2. 15:1,2. 01:1 respectively,and the phosphorylation of these protein kinases in KD group was significantly down - regulated(P < 0. 05). Conclusion ESCCAL 1 may regulate the EC9706 cell proliferation and metastasis through protein kinase phosphorylation.
Keywords：esophageal squamous cancerESCCAL 1protein kinase phosphorylation
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 1731-1733 )