Formononetin regresses HIF -1α/CXCR4 signaling and cell proliferation and migration in breast cancer cells
Ge Xin
Li Lin
Wang Fang
Guo Guangcheng
Li Jingruo
Gu Yuanting
Abstract：Objective To investigate the effect of formononetin on breast cancer cell behavior as well as its mechanism. Methods MDA -MB -231 cells were exposed to different doses (5,1 0,1 5,20 μM)of formononetin for 48 h respectively. The expression of hypoxia -inducible factor -1 α(HIF -1 α)mRNA and CXC chemokine receptor 4 (CXCR4)mRNA were an-alyzed by qRT -PCR.The expression of HIF -1 αand CXCR4 protein were analyzed by Western blot method.Furthermore, pShuttle -HIF -1 αoverexpression vector was constructed and transfected into MDA -MB -231 cells.Cell proliferation was de-termined by MTT assay.Cell migration was evaluated by Transwell assay.Results Compared with the control,exposure of for-mononetin significantly decreased both mRNA and protein expression of HIF -1 αand CXCR4 (P <0.05)in a dose dependent way.Overexpression of HIF -1 αraised HIF -1 αand CXCR4 expression in cancer cells after formononetin treatment (P <0.05),promoting cell proliferation (P <0.05)and enhancing cell migration (P <0.05).Conclusion Formononetin might af-fect breast cancer cell proliferation and migration through modulation of HIF -1 α/CXCR4 signaling transduction.
Keywords：formononetinbreast cancerhypoxia -inducible factor -1αCXC chemokine receptor 4proliferationmigration
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 1542-1544,1545 )