The effect and mechanism of nerve growth factor intervention on apoptotic neuron in hippocampus of immature SD rats after status epilepticus
Zhang Wenqian
Li Zhenbiao
Luo Qiang
He Ningxin
Zhao Qiongdan
Li Wanwan
Fan Caifang
Abstract：Objective To explore the effect and mechanism of nerve growth factor (NGF)by testing the expression of Caspase3 and Caspase9 in the hippocampus of immature SD rats after status epilepticus(SE).Methods Lithium -pilocarpine model of SE in immature SD rats were established.The apoptotic neuron was detected by TUNEL (TdT -mediated dUTP Nick End Labeling),and the expression of Caspase3 and Caspase9 were detected by immunohistochemical method.Results In nor-mal group,TUNNEL -positive cells,Caspase3 and Caspase9 expressed only a small amount at each time point.In SE group, the positive cells increased significantly at all observed points,and reached peak at 72 h.The positive cells in the NGF group re-duced significantly at each time point (except 4 h,P <0.05).The expression of activated Caspase3 and Caspase9 protein reached peak at 24 h.Compared with the SE group,the expression of acti-vated Caspase3 and Caspase9 protein revealed a decrease in the NGF group at 24 h and 72 h (P <0.01 ).Conclusion SE may lead to rats hippocampal neuronal apoptosis,and the neuronal apoptosis increases with time.Activated Caspase3 and Caspase9 can aggravate neuronal apoptosis.NGF can reduce the apoptosis of nerve cell and protect the brian.
Keywords：status epilepticusapoptosisCaspase3Caspase9nerve growth factor
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 1539-1541 )